By B. Moskowitz
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1979, 1980). I now present a revised version that takes into account the recent finding that PBLs cycling at the time of biopsy can appear as TG-resistant (TGr) variants. These cells, known as "phenocopies", may have resulted in overestimation of variant frequencies determined for normal individuals and those at risk. J. Albertini, personal communication). 1 ml heparin (1000 units/ml for injection without preservative) per 10 ml blood and mixed 1:1 with Hanks' balanced salt solution to which 2% heparin and 1% penicillin/strepto~cin (100 units/ 100 ~g/ml) have been added (HBSS).
Solid line represents the expected recovery frequencies. THE STRAUSS-ALBERTINI TEST 39 The experimental variant frequencies were near to theoretical expectations except in the case of the culture containing 10- 5 L-N cells. 5 x 10- 5). The sum of spontaneous and added Ter PBL frequencies accounted for the apparent discrepancy. The results of this experiment suggested that selection against the mutant phenotype did not occur in vitro and did not decrease efficiency of recovery in the present system.
Figure 7 is a schematic of the clinical course of an individual who was grafted with a cadaveric kidney, which failed due to chronic rejection. These data are representative of results from 20 individuals we have tested who suffered similar fates. We also have studied individuals who were fortunate to have received well-matched grafts from living related donors. Although variant frequency always increased shortly after grafting, it gradually returned to only moderate elevations. The present system may therefore be developed for use in monitoring the efficacy of azathioprine as an immunosuppressant for transplantation.