By Ursula K Le Guin
The primary of Simultaneity is a systematic leap forward in order to revolutionize interstellar civilization through making attainable instant communique. it's the lifestyles paintings of Shevek, a super physicist from the arid anarchist global of Anarres. yet Shevek's paintings is being stifled by means of jealous colleagues, so he travels to Anarres's sister-planet Urras, hoping to discover extra liberty and tolerance there. yet he quickly unearths himself getting used as a pawn in a perilous political video game.
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Benzonase (Sigma). DNAse I: 10 mg/mL in H2O. 0), 1% (w/v) SDS. Store at room temperature. Proteinase K (Sigma). Glycogen (Invitrogen, UK). 3 M Na3 citrate. Hybridization buffer (Amersham, UK). 5× SSC). Store at 4°C. 1×). 1. De novo synthesis of functional dystrophin molecules. Dystrophin is a rod-like protein of 3,685 amino acids (aa) localized beneath the inner surface of muscle cell membrane. It functions through four major structural domains: a N-terminal domain (1-756 aa), a central rod domain (757-3122 aa), a cysteine-rich (CR) domain (3123-3409 aa), and a distal C-terminal domain (3410-3685 aa).
Methods Mol Biol 290, 281–304. 22. Sternberg, E. , Perry, W. , and Olson, E. N. (1988) Identification of upstream and intragenic regulatory elements that confer cell-type-restricted and differentiation-specific expression on the muscle creatine kinase gene. Mol Cell Biol 8, 2896–2909. 23. Johnson, J. , Wold, B. , and Hauschka, S. D. (1989) Muscle creatine kinase sequence elements regulating skeletal and cardiac muscle expression in transgenic mice. Mol Cell Biol 9, 3393–3399. 24. Jaynes, J. , Johnson, J.
Full sequence data and corresponding files are normally provided in CD-ROM or e-mail for the customer following the final assembly of the de novo synthesized oligonucleotide sequences after verification of the final construct by sequencing. De novo synthesized species/codonoptimized cDNAs were supplied by GENEART as lyophilized plasmid material, reconstituted in water upon arrival and further downstream processed as required. Gene optimization and gene synthesis services are normally based on a pure fee-for-service model.